Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA-nogalamycin interactions.

M Egli1, L D Williams, C A Frederick

  • 1Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

Biochemistry
|February 5, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Access to and quality of care for sexual and gender minority women living with HIV in Metro Vancouver, Canada: Results from a longitudinal cohort study.

Women's health (London, England)·2023
Same author

Human papillomavirus and oral and oropharyngeal carcinoma: the essentials.

Australian dental journal·2018
Same author

Reasons of repeat dental treatment under general anaesthesia: A retrospective study.

European journal of paediatric dentistry·2018
Same author

A clinical trial of a novel emulsion for potential use as a saliva substitute in patients with radiation-induced xerostomia.

Journal of oral rehabilitation·2017
Same author

Does age matter? Sexual event-level analysis of age-disparate sexual partners among gay, bisexual and other men who have sex with men (GBM) in Vancouver, Canada.

Sexually transmitted infections·2016
Same author

Awareness and use of nonoccupational post-exposure prophylaxis among men who have sex with men in Vancouver, Canada.

HIV medicine·2016
Same journal

Aromatic Cage-Directed Azide-Methyllysine Photochemistry for Profiling Nonhistone Interacting Partners of the MeCP2 Methyl-CpG-Binding Domain.

Biochemistry·2026
Same journal

Differential Hydroxypyruvate Processing by <i>E. coli</i> and <i>P. aeruginosa</i> DXP Synthases Reveals Preferential Xylulose 5-Phosphate Formation by the <i>P. aeruginosa</i> Enzyme.

Biochemistry·2026
Same journal

Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

Biochemistry·2026
Same journal

Discovery of Bacterial Unspecific Peroxygenases.

Biochemistry·2026
Same journal

Lactate Biology: Subcellular Routing and Chemical Form Define Function.

Biochemistry·2026
Same journal

Nature's Anaerobic Toolkit: Glycyl Radical Enzymes and Their Expanding Functional and Mechanistic Diversity.

Biochemistry·2026
See all related articles

Nogalamycin, an anthracycline antibiotic, forms a complex with DNA requiring significant structural changes in both molecules. This interaction reveals DNA

Area of Science:

  • Structural biology
  • Molecular pharmacology
  • Biochemistry

Background:

  • Anthracycline antibiotics like nogalamycin are crucial in cancer therapy.
  • Nogalamycin's unique structure, resembling a dumbbell, differs significantly from daunomycin.
  • Understanding nogalamycin-DNA interactions is key to developing novel therapeutics.

Purpose of the Study:

  • To elucidate the X-ray crystal structure of the nogalamycin-DNA complex.
  • To analyze the molecular interactions and conformational changes upon complex formation.
  • To investigate the binding mode and potential sequence specificity of nogalamycin.

Main Methods:

  • X-ray crystallography was employed to determine the structure of the nogalamycin-DNA complex.
  • The study utilized a specific DNA hexamer, d(me5CGTsAme5CG), with modified bases and linkages.

Related Experiment Videos

  • Structural analysis focused on the intercalation site, drug-DNA contacts, and conformational distortions.

    • Main Results:

    • Nogalamycin intercalates into the DNA at terminal CpG steps, interacting with both strands and grooves.
    • Formation of the complex necessitates substantial conformational adjustments in both nogalamycin and DNA.
    • Base pairs surrounding the intercalation site exhibit distorted planarity and sliding, deviating from standard B-DNA geometry.

    Conclusions:

    • The nogalamycin-DNA complex highlights the remarkable conformational flexibility of DNA.
    • Intercalation involves significant distortions, suggesting a less sequence-specific binding mechanism for nogalamycin.
    • Further studies are needed to explore secondary effects that might influence nogalamycin's binding preferences.