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Related Experiment Video

Updated: Jun 18, 2026

Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins

Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins

Published on: March 17, 2023

Red blood cell storage lesion.

Daryl J Kor¹, Camille M Van Buskirk, Ognjen Gajic

¹Mayo Clinic, Rochester, MN 55905, USA.

Bosnian Journal of Basic Medical Sciences

|November 17, 2009

Summary

This summary is machine-generated.

Red blood cell (RBC) transfusions face scrutiny due to storage-induced changes, known as the storage lesion. These alterations affect oxygen delivery and immune function, impacting transfusion efficacy and safety.

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Area of Science:

Transfusion Medicine
Hematology
Immunology

Background:

Red blood cell (RBC) transfusion efficacy and safety are under increased review.
Storage of RBCs leads to biochemical, biomechanical, and immunologic alterations, termed the storage lesion.
These changes are linked to reduced tissue oxygenation and adverse transfusion effects.

Purpose of the Study:

To review alterations in RBCs and storage media during preservation.
To discuss the potential clinical consequences of the RBC storage lesion.

Main Methods:

Literature review of studies on RBC preservation and storage lesion.
Analysis of biochemical, biomechanical, and immunologic changes in stored RBCs.
Examination of correlations between storage lesion components and clinical outcomes.

Main Results:

Storage lesion involves decreased 2,3-diphosphoglycerate, ATP, and nitric oxide, impairing RBC deformability and oxygen delivery.
Accumulation of mediators like sCD40L, lyso-PC, and RANTES affects recipient immune function.
Storage lesion components are associated with transfusion-related adverse events.

Conclusions:

The RBC storage lesion significantly impacts transfusion outcomes.
Understanding these alterations is crucial for improving RBC transfusion safety and efficacy.
Further research is needed to mitigate the adverse effects of the storage lesion.