Variation in the composition of the AP1 complex in PC12 cells following induction by NGF and TPA

The rat pheochromocytoma cell line PC 12 differentiates in response to NGF. Exposure to NGF induces a class of genes termed immediate early that includes many transcription factors including c-jun and c-fos which can constitute the AP1 complex. Induction of such transcription factors by NGF could be a method by which the cell redirects its program of gene expression that results in differentiation. In this study, it is demonstrated that the complement of transcription factors that constitute the AP1 complex alters with the continued passage of PC12 cells. PC 12 cells from early passage contain no AP1 activity, whereas with passage the cells constitutively express an AP1 complex; however, no morphological differences are observed. The AP1 binding activity can be further induced in all PC12 cells studied by NGF or TPA. The analysis of c-jun, c-fos, and the fos-related antigens that can constitute the AP1 complex demonstrated compositional variation of this complex by passage in culture and by exposure to NGF or TPA. As these AP1 transcription complexes may mediate the action of NGF in PC12 cells it is important to correlate the changes in composition of the complex with differentiation.