Liposome characterization by quartz crystal microbalance measurements and atomic force microscopy

This chapter reviews liposome characterization by quartz crystal microbalance (QCM) measurements and atomic force microscopy (AFM). In many studies, AFM imaging is simply used to image liposomes with resolution often that does not allow morphological analysis. Although liposome size can be obtained by processing AFM images, it is found that liposomes flatten upon surface adsorption or immobilization. Liposome stability and stiffness have been characterized by using AFM imaging or AFM force measurements, although the latter method, using a microsphere attached on the AFM cantilever, seems more appropriate to limit liposome damage and to obtain more quantitative analysis, such as the Young's modulus. Investigation of liposome layers by QCM revealed that liposomes can be detected from a combined analysis of frequency and bandwidth shifts. However, QCM by itself provides only limited information on liposomes. QCM can be used to assess the presence of a layer and also to discriminate between rigid and viscoelastic ones. Liposome properties have been derived from QCM curves, but often this requires making hypotheses that are difficult to assess. AFM and QCM analyses need to be combined with other techniques to provide complementary information.