Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 18, 2026

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
08:35

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

Published on: February 21, 2014

Mixing solutions in inkjet formed vesicles.

Thomas H Li1, Jeanne C Stachowiak, Daniel A Fletcher

  • 1Department of Mechanical Engineering, University of California, Berkeley, California, USA.

Methods in Enzymology
|November 17, 2009
PubMed
Summary

Microfluidic encapsulation efficiently loads biomolecules into unilamellar vesicles. This technique achieves a high encapsulated fraction of 79%, guiding future applications in medicine and research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From the Laboratory to the Field: Assessing the Usability of the NTDscope in the Tropical Rainforest Region of Gabon.

The American journal of tropical medicine and hygiene·2026
Same author

A balance between nucleating and elongating actin filaments controls deformation of protein condensates.

Science advances·2026
Same author

Endocytic accessory proteins assemble clathrin while simultaneously destabilizing protein condensates.

bioRxiv : the preprint server for biology·2026
Same author

Connexin 43-Enriched Vesicles Improve Synchronization in hiPSC-Derived Cardiomyocytes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

PROTAC internalization and target degradation require clathrin-mediated endocytosis.

bioRxiv : the preprint server for biology·2026
Same author

Programmable kinetic barcoding for multiplexed RNA detection with Cas13a.

Nature biomedical engineering·2026

Area of Science:

  • Biotechnology
  • Biophysics
  • Materials Science

Background:

  • Controlling vesicle and liposome contents is crucial for therapeutic and research applications.
  • Encapsulating biomolecules like proteins, DNA, and RNA within vesicles presents significant challenges.
  • Microfluidic encapsulation offers a promising solution for efficient biomolecule loading.

Purpose of the Study:

  • To detail the equipment and protocol for microfluidic encapsulation of mixtures into unilamellar vesicles.
  • To quantify the encapsulated fraction achieved by this microfluidic method.
  • To investigate the impact of process parameters on encapsulation efficiency.

Main Methods:

  • Utilizing a piezoelectrically driven liquid jet to deform a planar bilayer and form vesicles.

More Related Videos

Reactive Inkjet Printing and Propulsion Analysis of Silk-based Self-propelled Micro-stirrers
09:23

Reactive Inkjet Printing and Propulsion Analysis of Silk-based Self-propelled Micro-stirrers

Published on: April 26, 2019

Inkjet-printed Polyvinyl Alcohol Multilayers
05:11

Inkjet-printed Polyvinyl Alcohol Multilayers

Published on: May 11, 2017

Related Experiment Videos

Last Updated: Jun 18, 2026

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
08:35

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

Published on: February 21, 2014

Reactive Inkjet Printing and Propulsion Analysis of Silk-based Self-propelled Micro-stirrers
09:23

Reactive Inkjet Printing and Propulsion Analysis of Silk-based Self-propelled Micro-stirrers

Published on: April 26, 2019

Inkjet-printed Polyvinyl Alcohol Multilayers
05:11

Inkjet-printed Polyvinyl Alcohol Multilayers

Published on: May 11, 2017

  • Employing a fluid vortex generated by the jet for mixing encapsulated solutions.
  • Measuring the encapsulated fraction using a falling vesicle technique.

    • Main Results:

    • Achieved a high encapsulated fraction of 79% +/- 5% for mixtures within unilamellar vesicles.
    • Demonstrated that continuous nozzle flow and varying actuation voltage polarity do not significantly affect encapsulation efficiency.
    • Validated the effectiveness of the microfluidic encapsulation technique for loading diverse biomolecules.

    Conclusions:

    • Microfluidic encapsulation is an effective method for loading arbitrary biomolecule mixtures into unilamellar vesicles.
    • The technique demonstrates robustness regarding continuous flow and voltage polarity, simplifying process optimization.
    • This work provides valuable insights for advancing microfluidic vesicle formation and cargo loading for various applications.