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Enzyme engineering for enantioselectivity: from trial-and-error to rational design?
Linda G Otten1, Frank Hollmann, Isabel W C E Arends
1Delft University of Technology, Department of Biotechnology, Biocatalysis and Organic Chemistry Group, Julianalaan 136, 2628 BL Delft, The Netherlands. l.g.otten@tudelft.nl
Trends in Biotechnology
|November 17, 2009
Summary
Finding highly enantioselective enzymes is key for biocatalysis in organic chemistry. Recent advances improve enzyme library generation and screening, moving towards more rational design for industrial applications.
Area of Science:
- Biocatalysis
- Organic Chemistry
- Enzyme Engineering
Background:
- Tailored enzymes are essential for industrial biocatalysis.
- Enantioselectivity is a critical parameter for enzyme utility and process competitiveness.
Purpose of the Study:
- To present and discuss recent advances in enzyme library generation and screening.
- To evaluate future trends in enzyme evolution for biocatalysis.
Main Methods:
- Review of improved methods for generating enzyme diversity.
- Discussion of enhanced screening techniques for identifying enantioselective enzymes.
Main Results:
- Significant progress has been made in efficiently generating and screening enzyme libraries.
- New methods enhance the discovery of enzymes with high enantioselectivity.
Conclusions:
- Advances in library generation and screening accelerate the identification of suitable biocatalysts.
- Future directions point towards rational evolution over random methods for enzyme optimization.
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