Related Experiment Video
Updated: Jun 18, 2026
![Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F55858.jpg&w=3840&q=50)
Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
Published on: December 4, 2017
Insight into the dehydrogenase catalyzed redox reactions and electron discharge pattern during fermentative hydrogen
S Venkata Mohan1, S Srikanth, M Lenin Babu
1Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad 500 607, India. vmohan_s@yahoo.com
Abstract:
Dehydrogenase (DH) activity associated with bio-electrochemical behavior was analyzed for the first time to understand the redox reactions involved in fermentative hydrogen (H(2)) production process in concurrence with proton (H(+)) shuttling and electron (e(-)) discharge (ED) pattern. DH facilitates the availability of H(+) through redox reactions to make H(2). We have designed a comprehensive experimental study to evaluate the DH activity (H(+) shuttling) and ED to understand the biochemical process with the function of pH (5, 6, 7 and 8) and metabolic microenvironment [anaerobic, anoxic and aerobic (control)]. DH activity was observed to be higher during anaerobic operation suggesting the higher availability of H(+) and e(-) due to the inter-conversion of metabolites and the same was reflected in the voltammetry analysis. Higher H(2) production under anaerobic operation corroborated well with these findings. The DH activity associated with H(+) shuttling and ED was also correlated with the substrate degradation pattern.
More Related Videos
08:57Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
10:21Developing Photosensitizer-Cobaloxime Hybrids for Solar-Driven H2 Production in Aqueous Aerobic Conditions
Published on: October 5, 2019
Related Concept Videos
Fermentation
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Microbial Fermentation
Oxidation and Reduction of Organic Molecules
The removal of an electron from a molecule, results in a...
Redox Equilibria: Overview
Redox Reactions
Redox Reactions