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Updated: Jun 18, 2026

Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
Dicer knockdown induces fibronectin-1 expression in HEK293T cells via induction of Egr1
Kai-Fu Tang1, Guan-Bin Song, Yi-Song Shi
1Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, PR China.
Background:
Dicer is a multidomain ribonuclease III enzyme involved in the biogenesis of microRNAs (miRNAs) and small interfering RNAs (siRNAs); depletion of Dicer was found to impair the migration of endothelial cells.
Methods:
siRNA transfection, cell migration assay, real-time RT-PCR, chromatin immunoprecipitation, Western blotting, ELISA, caspase-3 activity assay, and annexin-V-FITC assay were utilized.
Results:
Knockdown of Dicer impairs the migratory capacity of HEK293T cells and induces fibronectin-1. The upregulation of fibronectin-1 is dependent on Egr1. Fibronectin-1/Dicer double-knockdown cells showed a marked increase in apoptosis compared with fibronectin-1 single knockdown cells.
Conclusions:
Decreased Dicer expression induces fibronectin-1 expression via an Egr1-dependent manner.
General Significance:
Our data suggest that upregulation of fibronectin-1 protects Dicer knockdown HEK293T cells against apoptosis.
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