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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Related Experiment Video

Updated: Jun 18, 2026

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
12:11

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Published on: October 23, 2009

Sensitive enzyme-biosensor based on screen-printed electrodes for Ochratoxin A.

M Asunción Alonso-Lomillo1, Olga Domínguez-Renedo, Liliana Ferreira-Gonçalves

  • 1Analytical Chemistry Department, Faculty of Sciences, University of Burgos, Burgos, Spain. malomillo@ubu.es

Biosensors & Bioelectronics
|November 17, 2009
PubMed
Summary
This summary is machine-generated.

A new biosensor using horseradish peroxidase immobilized in polypyrrole detects Ochratoxin A selectively. This method offers high reproducibility and sensitivity for mycotoxin analysis in food products.

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Last Updated: Jun 18, 2026

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Iridium Oxide-reduced Graphene Oxide Nanohybrid Thin Film Modified Screen-printed Electrodes as Disposable Electrochemical Paper Microfluidic pH Sensors

Published on: November 22, 2016

Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Food Science

Background:

  • Ochratoxin A (OTA) is a prevalent mycotoxin found in various food products, posing significant health risks.
  • Accurate and sensitive detection methods for OTA are crucial for food safety and regulatory compliance.
  • Biosensors offer a promising platform for rapid and selective mycotoxin determination.

Purpose of the Study:

  • To develop and optimize a novel biosensor for the selective detection of Ochratoxin A.
  • To immobilize horseradish peroxidase (HRP) in a polypyrrole matrix on screen-printed carbon electrodes for enhanced stability and performance.
  • To evaluate the biosensor's performance in terms of sensitivity, reproducibility, and applicability to real food samples.

Main Methods:

  • Horseradish peroxidase (HRP) was immobilized onto polypyrrole-modified screen-printed carbon electrodes.
  • Chronoamperometric detection was employed for Ochratoxin A determination.
  • Experimental design methodology was used to optimize key parameters: buffer pH, applied potential, and H(2)O(2) concentration.
  • Reproducibility and repeatability were assessed using calibration curve slopes.
  • Limit of detection (LOD) was determined under optimized conditions.

Main Results:

  • The developed biosensor demonstrated selective detection of Ochratoxin A with a limit of detection of 0.1 ng mL(-1).
  • Optimized conditions yielded high reproducibility (RSD 1.9%) and repeatability (RSD 7.1%).
  • The biosensor showed excellent performance in analyzing spiked beer and roasted coffee samples, with average recoveries of 103% and 99%, respectively, and RSD < 5%.

Conclusions:

  • The polypyrrole-immobilized HRP biosensor provides a reliable and sensitive platform for Ochratoxin A detection.
  • The experimental design approach effectively optimized the chronoamperometric determination of OTA.
  • The biosensor's viability in real food matrices highlights its potential for practical food safety applications.