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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
Published on: March 31, 2022
RecA-independent DNA damage induction of Mycobacterium tuberculosis ruvC despite an appropriately located SOS box
Lisa F Dawson1, Joanna Dillury, Elaine O Davis
1MRC National Institute for Medical Research, The Ridgeway, London NW7 1AA, UK.
Abstract:
Mycobacterium tuberculosis ruvC was induced by DNA damage in a DeltarecA strain despite having an appropriately positioned SOS box to which LexA binds in vitro. An inducible transcript start mapped within the SOS box, and transcriptional fusions identified the promoter. Disruption of the SOS box did not prevent induction, indicating that an alternative mechanism plays a significant role in the control of ruvC expression.
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