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Updated: Jul 15, 2026

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Identification of sulfurtransferase enzymes in Azotobacter vinelandii
S Pagani1, E Franchi, R Colnaghi
1Dipartimento di Scienze Molecolari Agroalimentari, University of Milan, Italy.
Abstract:
Rhodanese and 3-mercaptopyruvate sulphurtransferase have been identified in A. vinelandii. Two distinct active fractions of the two sulphur transferases were obtained after FPLC ion-exchange chromatography of material partially purified from crude extracts. Rhodanese has been purified to homogeneity, and it consists of one polypeptide chain of Mr ca 25,000. A partial purification of 3-mercaptopyruvate sulphurtransferase was obtained.
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