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Related Experiment Video

Updated: Jun 18, 2026

Transmission Electron Microscopy as the Visualization Technique for Analysis of Circadian Synaptic Plasticity in the Mouse Barrel Cortex
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Postsynaptic deregulation in GAP-43 heterozygous mouse barrel cortex.

Emily A Kelly1, Marie-Eve Tremblay, James S McCasland

  • 1Department of Neurobiology and Anatomy, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.

Cerebral Cortex (New York, N.Y. : 1991)
|November 17, 2009
PubMed
Summary

Reduced expression of GAP-43 in mice alters barrel cortex development, causing initial synaptic and dendritic defects. The brain shows adaptive compensation and partial recovery, highlighting GAP-43

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Synaptic Plasticity

Background:

  • Whisker-related barrel formation in the somatosensory cortex depends on thalamocortical afferent (TCA) and cortical neuron communication.
  • Growth-associated protein 43 (GAP-43) is vital for guiding TCAs but its role in neural communication remains unclear.

Purpose of the Study:

  • To investigate how reduced early expression of presynaptic GAP-43 affects postsynaptic differentiation of barrel cells.
  • To explore the impact of GAP-43 on TCA-cortical neuron interactions during development.

Main Methods:

  • Utilized GAP-43 heterozygous (HZ) mice to model reduced presynaptic GAP-43 expression.
  • Employed cytochrome oxidase staining, Golgi impregnation, and microtubule-associated protein 2 immunohistochemistry.
  • Assessed synaptogenesis through NR1 and GluR1 colocalization.

Main Results:

  • Observed transiently increased metabolic activity in the barrel cortex of HZ mice (P6-P14).
  • Detected anomalous dendritic patterning and length in HZ cortex at P5, with partial recovery by P7.
  • Identified early synaptogenesis defects (P4-P5) in HZ mice, normalizing by P7.

Conclusions:

  • Reduced early GAP-43 expression leads to abnormal postsynaptic differentiation during barrel development.
  • The developing brain exhibits adaptive compensation and partial rescue of the observed defects.
  • Findings suggest a critical role for GAP-43 in regulating early synaptic and structural development in the somatosensory cortex.