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Updated: Jun 18, 2026

Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements
Published on: July 29, 2021
Nitrate and nitrite in biology, nutrition and therapeutics
Jon O Lundberg1, Mark T Gladwin, Amrita Ahluwalia
1Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden. jon.lundberg@ki.se
Abstract:
Inorganic nitrate and nitrite from endogenous or dietary sources are metabolized in vivo to nitric oxide (NO) and other bioactive nitrogen oxides. The nitrate-nitrite-NO pathway is emerging as an important mediator of blood flow regulation, cell signaling, energetics and tissue responses to hypoxia. The latest advances in our understanding of the biochemistry, physiology and therapeutics of nitrate, nitrite and NO were discussed during a recent 2-day meeting at the Nobel Forum, Karolinska Institutet in Stockholm.
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