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Updated: Jun 18, 2026

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Non-Invasive Compression-Induced Anterior Cruciate Ligament (ACL) Injury and In Vivo Imaging of Protease Activity in Mice
Published on: September 29, 2023
Changes in ACL length at different knee flexion angles: an in vivo biomechanical study
Yon-Sik Yoo1, Woon-Seob Jeong, Nagraj S Shetty
1200-704 Department of Orthopaedic Surgery, College of Medicine, University of Hallym, Gyodong 153, Chuncheon, Republic of Korea. ybw1999@gmail.com
Summary
Anterior cruciate ligament (ACL) bundle lengths change with knee flexion. Understanding these length variations is crucial for anatomical ACL reconstruction and optimizing graft fixation during surgery.
Area of Science:
- Orthopedic Surgery
- Biomedical Engineering
- Anatomy
Background:
- Anatomical reconstruction of the anterior cruciate ligament (ACL) is a significant focus in orthopedic surgery.
- The double-bundle reconstruction technique for ACL injuries is increasingly advocated.
- Understanding the dynamic length changes of ACL bundles during knee motion is essential for surgical planning.
Purpose of the Study:
- To investigate how the lengths of individual anterior cruciate ligament (ACL) bundles change with varying knee flexion angles.
- To compare the length variations between different virtual ACL bundles (anatomical single bundle, anteromedial, posterolateral, and over-the-top).
Main Methods:
- High-resolution computed tomography (CT) scans were used to create 3D knee models from ten healthy subjects.
- Customized software facilitated digital length measurements of four virtual ACL bundles at fixed points on the femur and tibia.
- Length-versus-flexion curves were generated, and statistical analysis was performed for flexion angles of 0, 45, 90, and 135 degrees.
Main Results:
- All virtual ACL bundles reached their maximum length at full knee extension (0 degrees).
- The posterolateral bundle exhibited a significantly different length compared to other bundles at full extension.
- No significant length differences were observed between the anteromedial and over-the-top single bundles across all tested flexion angles.
Conclusions:
- Three-dimensional CT imaging is a viable method for assessing dynamic length changes in virtual ACL bundles.
- This approach enhances the understanding of ACL bundle function in clinical contexts, particularly for surgical reconstruction.
- Accurate assessment of bundle length variations can inform graft fixation strategies in ACL surgery.
