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A stability-indicating high-performance liquid chromatographic method for the determination of methocarbamol in
María A Rosasco1, Rita Ceresole, Clara C Forastieri
1Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Control de Calidad de Medicamentos, Junín 956, 1113 Buenos Aires, Argentina.
Abstract:
An isocratic HPLC method was developed and validated for the quantitation of methocarbamol in the presence of its degradation products. Quantitation was achieved using a reversed-phase C18 column at ambient temperature with mobile phase consisting of methanol-water-tetrahydrofuran (25 + 65 + 10, v/v). The flow rate was 0.9 mL/min. The detection was by UV light at 274 nm. The proposed method was validated for selectivity, precision, linearity, and accuracy. The assay method was found to be linear from 159.0 to 793.2 microg/mL (3.2 to 15.9 microg injected). All validation parameters were within the acceptable range. The developed method was successfully applied to estimate the amount of methocarbamol in a veterinary injection.
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