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Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation
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Dendrimers in gene transfection

D G Shcharbin1, B Klajnert, M Bryszewska

  • 1Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, Minsk, 220072, Belarus. d.shcharbin@mail.ru

Biochemistry. Biokhimiia
|November 18, 2009
PubMed

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Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...