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Updated: Jun 18, 2026

Apoplast-Extraction Based Method to Improve the Purity of Plant Produced Recombinant Protein
Published on: July 5, 2024
Production of biologically active human myelocytokines in plants
A S Zvereva1, L E Petrovskaya, A V Rodina
1Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia. zvereva@gmail.com
Abstract:
An effective system for expression of human granulocyte and granulocyte macrophage colony-stimulating factors (hG-CSF and hGM-CSF) in Nicotiana benthamiana plants was developed using viral vector based on tobacco mosaic virus infecting cruciferous plants. The genes of target proteins were cloned into the viral vector driven by actin promoter of Arabidopsis thaliana. The expression vectors were delivered into plant cells by agroinjection. Maximal synthesis rate was detected 5 days after injection and was up to 500 and 300 mg per kg of fresh leaves for hG-CSF and hGM-CSF, respectively. The yield of purified hG-CSF and hGM-CSF was 100 and 50 mg/kg of fresh leaves, respectively. Recombinant plant-made hG-CSF and hGM-CSF stimulated proliferation of murine bone marrow and human erythroleucosis TF-1 cells, respectively, at the same rate as the commercial drugs.
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