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Preparation and characterization of Cu,Zn-superoxide dismutase covalently modified by polyunsaturated fatty acids
1College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, P. R. China.
Abstract:
To improve its stability and lipophilicity, Cu,Zn-superoxide dismutase (SOD) was chemically modified with linoleic and alpha-linolenic acids using two different methods. Higher retained enzymatic activity has been observed compared with SOD modified by macromolecular substances. Enhanced heat stability, acid and alkali resistance, and anti-pepsin/trypsin ability of the modified SOD were observed compared with those of the natural enzyme, the apparent oil-water partition coefficient being especially increased. The results characterize SOD modified with polyunsaturated fatty acids as a promising pharmacological tool.
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