Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...

Regulation of Bacterial Virulence

Regulation of Bacterial Virulence

Pathogenic bacteria employ a range of regulatory mechanisms to modulate the expression of virulence genes in response to environmental and host-derived signals. These mechanisms ensure that virulence factors are expressed only under favorable conditions, thereby optimizing infection and survival strategies.Mechanisms of Virulence RegulationKey regulatory strategies include:Two-Component Systems: These consist of a membrane-bound sensor kinase and a cytoplasmic response regulator. Environmental...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Phage Intolerance Impacts Antibiotic Susceptibility and Virulence in Staphylococcus aureus.

Current microbiology·2026

Same author

A broader perspective on antimicrobial resistance in food chains.

npj antimicrobials and resistance·2026

Same author

Resveratrol-Derived Dimers With Antimicrobial and Immune-Enhancing Activities.

BioFactors (Oxford, England)·2026

Same author

Structure elucidation of leadbeaterin-1, an ichthyotoxin associated with fish-killing blooms of Chrysochromulina leadbeateri in Northern Norwegian fjords.

Harmful algae·2026

Same author

An Exploratory LC-HRMS Metabolomics Study of Culture Medium-Dependent Metabolic Variation and Bioactivity in Ten Fungal Strains.

International journal of molecular sciences·2026

Same author

A novel therapeutic strategy of methicillin-resistant <i>Staphylococcus aureus</i>.

Engineering microbiology·2026

Search research articles

Related Experiment Video

Updated: Jun 18, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

Method for screening compounds that influence virulence gene expression in Staphylococcus aureus.

Anita Nielsen¹, Kristian F Nielsen, Dorte Frees

¹Department of Veterinary Disease Biology, Stigboejlen 4, 1870 Frederiksberg C, Denmark.

Antimicrobial Agents and Chemotherapy

|November 18, 2009

Summary

We developed a simple assay to screen for new antivirulence compounds targeting Staphylococcus aureus. This method detects changes in virulence gene expression, aiding the discovery of novel therapeutics against this pathogen.

More Related Videos

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

Published on: January 3, 2020

Related Experiment Videos

Last Updated: Jun 18, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

Published on: March 28, 2017

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

Published on: January 3, 2020

Area of Science:

Microbiology
Molecular Biology
Drug Discovery

Background:

Staphylococcus aureus is a major human pathogen responsible for various infections.
Controlling virulence factors is a key strategy for developing new anti-infective therapies.
The agr locus plays a crucial role in regulating Staphylococcus aureus virulence.

Purpose of the Study:

To develop a simple and effective assay for screening compounds that modulate virulence gene expression in Staphylococcus aureus.
To identify novel antivirulence compounds using this assay.

Main Methods:

Utilized transcriptional reporter strains of Staphylococcus aureus with lacZ fused to key virulence genes.
Employed a chromogenic beta-galactosidase substrate to detect changes in reporter gene activity.
Scored compounds based on color change, indicating effects on virulence gene expression and agr locus activity.

Main Results:

Successfully established a straightforward assay for assessing compound effects on virulence gene expression.
Demonstrated the assay's capability to screen for antivirulence compounds, including those derived from fungal sources.

Conclusions:

The developed assay is a valuable tool for discovering novel antivirulence compounds against Staphylococcus aureus.
This screening method facilitates the identification of compounds that target essential virulence pathways.