Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An autosampling microfluidic chip enables FEN1-aided recombinase polymerase amplification to rapidly detect multiple urinary tract infection pathogens on-site.

Mikrochimica acta·2026

Same author

The influence of empathy with nature on pro-environmental behaviors in young children: evidence from behavioral and eye-tracking studies.

Frontiers in psychology·2026

Same author

The "balance point" of serum creatinine: methodological concerns on peak timing and selection bias.

Pediatric nephrology (Berlin, Germany)·2026

Same author

Epidemiological characteristics of elderly emergency visits in Wenzhou.

BMC emergency medicine·2026

Same author

Characterization of ceftazidime-avibactam resistant blaKPC-35-harbouring Klebsiella pneumoniae ST15 from bloodstream infection.

Acta microbiologica et immunologica Hungarica·2026

Same author

Photo-initiated droplet digital recombinase polymerase amplification coupled with invasive reactions enables ultra-fast antimicrobial susceptibility testing.

Biosensors & bioelectronics·2026

Search research articles

Related Experiment Video

Updated: Jun 18, 2026

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

A gel-based solid-phase amplification and its application for SNP typing and sequencing on-chip.

Huan Huang¹, Pengfeng Xiao, Zongtai Qi

¹Huadong Research Institute for Medicine and Biotechnics, Nanjing, 210002, China.

|November 18, 2009

Summary

This study introduces 3D gel-based solid-phase amplification (SPA) for higher DNA amplification capacity. This novel method enables accurate genotyping and reusable amplicons for parallel detection.

More Related Videos

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

Related Experiment Videos

Last Updated: Jun 18, 2026

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

Infinium Assay for Large-scale SNP Genotyping Applications

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

Area of Science:

Biotechnology
Molecular Biology
Genomics

Background:

Conventional 2D solid-phase amplification (SPA) is limited by low primer immobilization capacity.
This limitation restricts the overall amplification efficiency in nucleic acid analysis.

Purpose of the Study:

To develop a 3D SPA method with enhanced amplification capacity using hydrogel immobilization.
To demonstrate the utility of gel-based SPA for accurate genotyping and downstream applications.

Main Methods:

Primers were modified and copolymerized into a polyacrylamide hydrogel attached to a glass slide.
A one-step nucleic acid immobilization process was optimized using N,N,N',N'-tetramethylethylenediamine (TEMED).
Gel-based SPA products were analyzed using dual-color fluorescence hybridization, BAMPER, and pyrosequencing.

Main Results:

The 3D gel-based SPA achieved significantly higher amplification capacity compared to conventional methods.
Accurate genotype discrimination was achieved using the developed gel-based SPA.
Pyrosequencing and reusable amplicons for parallel detection were successfully demonstrated.

Conclusions:

The proposed 3D gel-based SPA offers a powerful platform for high-capacity nucleic acid amplification.
This method facilitates direct use of on-chip amplicons for various parallel detection assays.
The strong DNA-gel linkage allows for reusable amplicons, enhancing assay efficiency and cost-effectiveness.