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Related Experiment Video

Updated: Jun 18, 2026

Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric Analysis

Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric Analysis

Published on: July 26, 2024

Spatial (Tbata) expression in mature medullary thymic epithelial cells.

Murielle Saade¹, Magali Irla, Miriam Yammine

¹INSERM U928, Technologies Avancées pour le Génome et la Clinique, Université de la Méditerranée, Parc Scientifique de Luminy, Marseille Cedex, France.

European Journal of Immunology

|November 18, 2009

Summary

Spatial gene expression in thymic epithelial cells (TEC) was characterized for the first time. Spatial is regulated by Aire, crucial for thymus organogenesis and function.

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Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

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Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

Published on: August 8, 2014

Area of Science:

Immunology
Developmental Biology
Molecular Biology

Background:

The Spatial gene is known to be expressed in specific polarized cells like germ cells and neurons.
Its role and expression patterns within the thymus, particularly in thymic epithelial cells (TEC), remain poorly understood.

Purpose of the Study:

To comprehensively characterize the expression of the Spatial gene in TEC throughout mouse thymus development and in adult stages.
To investigate the regulation of Spatial gene expression by thymocyte-derived signals and key transcription factors.

Main Methods:

Expression analysis of Spatial in TEC during embryonic and adult thymus ontogeny.
Utilizing genetically modified mouse models with blocked thymocyte development.
Investigating Spatial expression in purified thymic epithelial cell subsets.
Analyzing Spatial expression in Aire- and RANKL-deficient mice.

Main Results:

Spatial expression is detected in TEC as early as embryonic day E10.5 and persists in various TEC populations, including progenitors.
Spatial expression in TEC during thymus organogenesis is independent of thymocyte signals.
Spatial short and long isoforms are found in both cortical TEC (cTEC) and mature medullary TEC (mTEC).
Spatial exhibits nuclear localization specifically in mature mTEC expressing UEA1 and Aire.
Spatial expression is significantly reduced in Aire- and RANKL-deficient mouse thymus.

Conclusions:

Spatial gene expression is a critical feature of thymic epithelial cells throughout development.
Aire plays a significant role in regulating Spatial gene expression in the thymus.
Spatial's expression pattern and regulation suggest a role in thymus function and potentially promiscuous gene expression.