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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Pre-mRNA Processing

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...

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Related Experiment Video

Updated: Jun 18, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

The pathobiology of splicing.

Amanda J Ward¹, Thomas A Cooper

¹Departments of Molecular and Cellular Biology and Pathology, Baylor College of Medicine, Houston, TX 77030, USA.

The Journal of Pathology

|November 18, 2009

Summary

Gene splicing, a process where introns are removed from precursor mRNAs (pre-mRNAs) to form mature messenger RNAs (mRNAs), enables alternative splicing for gene expression diversity. Dysregulation of this crucial process can lead to various diseases.

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Most human genes are discontinuous, composed of exons and introns.
Gene expression involves transcribing DNA into precursor messenger RNAs (pre-mRNAs).
Pre-mRNAs undergo splicing to remove introns and join exons, forming mature messenger RNAs (mRNAs).

Purpose of the Study:

To explain the mechanism and significance of alternative splicing in generating mRNA diversity.
To elucidate the role of regulatory elements and protein factors in guiding the splicing process.
To highlight the impact of splicing misregulation on human diseases and therapeutic strategies.

Main Methods:

Analysis of gene structure and the process of co-transcriptional splicing.
Identification of cis-regulatory elements and trans-acting factors involved in splicing.

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Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Last Updated: Jun 18, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Review of mutation impacts on splicing fidelity and disease association.

Main Results:

Alternative splicing allows a single gene to produce multiple mRNA variants.
Specific sequences within introns and exons, along with auxiliary proteins, direct spliceosome activity.
Mutations in regulatory elements or splicing factors can disrupt normal splicing, leading to disease.

Conclusions:

Splicing and alternative splicing are fundamental to gene expression regulation and protein diversity.
Aberrant splicing is implicated in the pathogenesis of numerous diseases.
Understanding splicing mechanisms offers potential for novel therapeutic interventions in genetic disorders.