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Related Concept Videos

Autophagy01:27

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...

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Related Experiment Video

Updated: Jun 18, 2026

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
11:39

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

Measuring autophagy in macrophages.

James Harris1, Orla Hanrahan1, Sergio A De Haro2

  • 1School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland.

Current Protocols in Immunology
|November 18, 2009
PubMed
Summary
This summary is machine-generated.

Macroautophagy, a cellular process, aids survival by recycling components during starvation and removing damaged parts. New methods allow measuring this vital autophagy process in immune cells like macrophages.

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Related Experiment Videos

Last Updated: Jun 18, 2026

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
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Published on: July 21, 2017

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
09:59

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Published on: May 3, 2013

Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages
06:47

Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages

Published on: May 24, 2019

Area of Science:

  • Cell Biology
  • Immunology
  • Molecular Biology

Background:

  • Macroautophagy is a fundamental cellular mechanism for degrading and recycling cytoplasmic components.
  • It plays a critical role in maintaining cellular homeostasis, promoting cell survival during nutrient deprivation.
  • Emerging evidence highlights autophagy's significant involvement in immune responses, particularly in macrophages and dendritic cells against bacterial pathogens.

Purpose of the Study:

  • To present reliable protocols for quantifying macroautophagy in macrophages.
  • To provide researchers with tools to investigate the role of autophagy in immune cell function.

Main Methods:

  • Detailed experimental procedures for measuring autophagy flux in macrophage cell models.
  • Techniques may include Western blotting for autophagy markers (e.g., LC3-II), tandem fluorescent-tagged reporters, and lysosomal activity assays.

Main Results:

  • Established protocols enable accurate assessment of autophagic activity in macrophages.
  • The methods are suitable for studying how autophagy is modulated during infection or other immune stimuli.

Conclusions:

  • The presented protocols are essential for advancing the understanding of autophagy's role in macrophage immunity.
  • Accurate measurement of autophagy in macrophages is crucial for dissecting its contribution to host defense against bacterial pathogens.