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Published on: January 5, 2024
[Empirical study of oridonin-induced gastric cancer cells MKN45 apoptosis]
Xu-jun He1, Hui-ju Wang, Yin-jie Xia
1Key Laboratory of Gastroenterology of Zhejiang Province, Zhejiang Provincial People's Hospital, Hangzhou 310014, China.
Objective:
To investigate the growth inhibition and apoptosis of gastric cancer cell MKN45 induced by oridonin and its mechanism.
Methods:
The MTT method was used to investigate the inhibitory effect of oridonin on MKN45 cells. The AO/EB and Hoechst 33258 staining were used to observe the cell morphologic changes of apoptosis induced by oridonin. Prophase apoptotic ratio and cell cycle change were evaluated by GuavaEasycyte PCA-96 system. The expressions of Bcl-2, Bax and caspase 3 proteins were determined by Western blot.
Results:
Oridonin significantly inhibited the proliferation of MKN45 cells in dose- and time-dependent manner. Typical apoptotic features of the cells treated with oridonin were found by AO/EB and Hoechest33258 staining. When MKN45 cells were treated with different doses of oridonin for 12 h, the prophase apoptotic ratio was stepped up from 3.3% (untreated group) to 8.7%-17.9%; after 24 h, from 4.8% (untreated group) to 13.9%-29.3%. There was significant difference between treated and untreated groups (P <0.01). After treatment with oridonin for 24 h, MKN45 cells were arrested at G(2)/M phase. Western blot analysis showed up-regulated expression of Bax and caspase-3, and no significant change of Bcl-2, but Bcl-2/Bax ratio decreased significantly.
Conclusions:
Oridonin significantly inhibits the proliferation of MKN45 cell. Apoptosis of MKN45 induced by oridonin may be associated with the up-regulated expression of Bax and the change of Bcl-2/Bax ratio, thus to activate the caspase pathway.
Insights
Oridonin effectively inhibits gastric cancer cell growth by inducing apoptosis. This occurs through increased Bax and caspase-3 expression, altering the Bcl-2/Bax ratio and activating cell death pathways.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Context:
- Gastric cancer remains a significant global health challenge.
- Identifying novel therapeutic agents is crucial for improving patient outcomes.
- Oridonin, a compound from Rabdosia rubescens, shows potential anticancer properties.
Purpose:
- To investigate the growth inhibitory and apoptosis-inducing effects of oridonin on MKN45 gastric cancer cells.
- To elucidate the underlying molecular mechanisms of oridonin-induced apoptosis.
Summary:
- Oridonin significantly inhibited MKN45 cell proliferation in a dose- and time-dependent manner.
- Apoptosis was confirmed through morphological changes and increased apoptotic ratios.
- Oridonin treatment led to G2/M cell cycle arrest.
- Western blot analysis revealed upregulated Bax and caspase-3 expression, and a decreased Bcl-2/Bax ratio.
Impact:
- These findings suggest oridonin's potential as a therapeutic agent for gastric cancer.
- The study provides mechanistic insights into oridonin's anti-cancer activity.
- Further research may explore oridonin's clinical applicability in gastric cancer treatment.
