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Updated: Jun 18, 2026
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
Published on: October 11, 2015
[siRNAs targetting sphingomyelin phosphodiesterase 1 protect mouse oocytes from apoptosis]
Ren-Li Zhang1, Jin-Xiu Meng, An-Ming Wen
1Center of Reproductive Medicine, Guanddong Academy of Medical Sciences (Guangdong Provincial People's Hospital), Guangzhou 510080, China. renlizhangm@yahoo.com.cn
Objective:
To investigate the potential of siRNAs targeting sphingomyelin phosphodiesterase 1 (SMPD1) in protecting the oocytes from apoptosis, and explore new approaches to female fertility preservation.
Methods:
Chemically synthesized siRNA targeting SMPD1 were introduced into mouse oocytes retrieved by hyperstimulation, and the cell apoptosis was analyzed by comic assay 48 and 72 h later.
Results:
In the oocytes without any siRNA injection, oocyte DNA damage occurred after 24 h, and large amount of DNA fragments migrated from the cells 48 h later. In oocytes injected with siRNA003, DNA migration decreased significantly as compared with the control and the other two groups injected with siRNA001 and siRNA002 (P<0.01).
Conclusion:
siRNA targeting SMPD1 may protect the oocytes from apoptosis, and has the potential for use in future female fertility preservation.
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