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Updated: Jun 18, 2026
Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines
Published on: August 21, 2019
[Comparison of DNA-dependent protein kinase catalytic subunit expression in two lung adenocarcinoma cell lines with
Wei-Jian Cen1, Yi Pan, Wei-Xiong Li
1Department of Radiation Oncology, Guangdong Provincial People's Hospital, Guangzhou 510080, China.
Objective:
To investigate DNA-dependent protein kinase catalytic subunit (DNA-PKcs) content and activity in lung adenocarcinoma cell lines and its correlation with radiosensitivity.
Methods:
The content and activity of DNA-PKcs were analyzed in two lung adenocarcinoma cell lines A549 and H1299 by Western blotting and the Signa TECT DNA-PK assay kit. The dose-survival relationship for two cell lines was analyzed using clonogenic formation assay.
Results:
A549 was more radiosensitive than H1299. The survival fractions at 2 Gy (SF2) were 0.7412 in A549 cell line and 0.2473 in H1299 cell line. The content of DNA-PKcs was significantly higher in A549 cells than in H1299 cells (t=10.37, P<0.001). The integrated optical densities were 3.29-/+0.44 in A549 cells and 0.50-/+0.17 in H1299 cells. DNA-PKcs activities in A549 and H1299 cells were 8.29-/+1.37 and 2.47-/+1.09, respectively, showing a significant difference between them (t=5.76, P=0.005).
Conclusion:
DNA-PKcs is an important factor to affect the radiosensitivity of lung adenocarcinoma cell lines.
