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Related Experiment Video

Updated: Jun 18, 2026

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4⁺ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

Viruses within the Flaviviridae decrease CD4 expression and inhibit HIV replication in human CD4+ cells.

Jinhua Xiang¹, James H McLinden, Robert A Rydze

¹Department of Internal Medicine, Iowa City Veterans Affairs Medical Center and University of Iowa, Iowa City, IA 52242, USA.

Journal of Immunology (Baltimore, Md. : 1950)

|November 20, 2009

Summary

This summary is machine-generated.

Flavivirus NS5 proteins, including from Dengue and Yellow Fever viruses, inhibit HIV replication by reducing CD4 gene expression. This discovery may offer new therapeutic strategies for HIV and immune diseases.

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A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses

Published on: August 31, 2014

Area of Science:

Virology
Immunology
Molecular Biology

Background:

Viral coinfections can alter host cell functions, impacting immune evasion and microbial replication.
Certain flaviviruses, like Dengue virus (DV) and GB virus type C (GBV-C), are linked to reduced HIV replication and improved survival in HIV-infected individuals.

Purpose of the Study:

To investigate if the inhibitory effect on HIV replication is conserved across various flaviviruses.
To characterize the mechanisms by which flaviviruses, specifically their NS5 proteins, inhibit HIV replication.

Main Methods:

Expression of NS5 proteins from GBV-C, DV, Hepatitis C virus, West Nile virus, and Yellow Fever virus (YFV) in CD4(+) T cells.
Infection of CD4(+) T cells and macrophages with YFV (17D vaccine strain).
Measurement of HIV replication, CD4 mRNA levels, and cell surface CD4 protein expression.

Main Results:

All tested flavivirus NS5 proteins inhibited HIV replication in CD4(+) T cells.
This inhibition correlated with decreased steady-state CD4 mRNA and reduced cell surface CD4 protein expression.
YFV infection also inhibited HIV replication and decreased CD4 gene expression in relevant cell types.

Conclusions:

Flavivirus NS5 proteins consistently decrease CD4 gene expression.
Flavivirus-mediated CD4 regulation may impact innate and adaptive immunity, contributing to HIV replication inhibition.
Understanding these mechanisms could lead to novel therapeutic strategies for HIV and immunological disorders.