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Updated: Jun 18, 2026
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
Rapid detection of aac(6')-Ib-cr quinolone resistance gene by pyrosequencing
Thomas Guillard1, Véronique Duval, Hélène Moret
1Laboratoire de Bactériologie-Virologie-Hygiène, CHU Robert Debré, Rue du Général Koenig, 51092 Reims cedex, France. tguillard@chu-reims.fr
Abstract:
Pyrosequencing was used to rapidly detect aac(6')-Ib and aac(6')-Ib-cr genes. This plasmid-mediated quinolone resistance determinant is increasing in extended-spectrum beta-lactamase-producing Enterobacteriaceae. This method is faster and more cost-effective than the methods previously described. Sequences obtained with this pyrosequencing method showed 100% concordance with conventional sequencing.
