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Aerobic batch cultivation in micro bioreactor with integrated electrochemical sensor array.

Michiel van Leeuwen1, Erik E Krommenhoek, Joseph J Heijnen

  • 1Dept. of Biotechnology, Delft University of Technology and Kluyver Centre for Genomics of Industrial Fermentation, Julianalaan 67, 2628 BC, Delft, The Netherlands.

Biotechnology Progress
|November 20, 2009
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Summary

This study demonstrates the use of micro bioreactors with electrochemical sensors for monitoring parallel cultivations of *Candida utilis*. The system provides reproducible online measurements comparable to traditional bioreactors, enabling high-throughput fermentation analysis.

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Area of Science:

  • Biotechnology
  • Microbial Cultivation
  • Biosensing Technology

Background:

  • High-throughput screening and process monitoring are crucial in microbial biotechnology.
  • Conventional bioreactors are limited in parallel processing capabilities.
  • Microscale cultivation systems offer potential for increased throughput but require robust monitoring tools.

Purpose of the Study:

  • To evaluate the applicability of electrochemical sensor arrays for online monitoring of parallel micro-scale fermentations.
  • To assess the reproducibility and comparability of micro-bioreactor data with conventional bench-scale systems.
  • To demonstrate the integration of microfluidic devices with biosensing for enhanced microbial cultivation.

Main Methods:

  • Aerobic batch cultivations of *Candida utilis* were performed in 100 muL micro bioreactors.
  • Micro bioreactors were equipped with electrochemical sensor arrays for real-time measurement of temperature, pH, dissolved oxygen, and viable biomass.
  • Carbon dioxide (CO2) production rate was determined via online measurement of cumulative CO2 production.

Main Results:

  • Electrochemical sensor arrays provided reproducible online measurements of key cultivation parameters.
  • Data from micro bioreactors were highly comparable to results from a conventional 4L bench-scale bioreactor.
  • The system demonstrated compatibility with high-throughput cultivation formats, such as 96-well microtiter plates.

Conclusions:

  • Electrochemical sensor arrays are applicable for monitoring parallel micro-scale fermentations.
  • This technology enables robust, reproducible, and comparable data acquisition in microfluidic cultivation systems.
  • The developed system shows promise for advancing high-throughput microbial process development and analysis.