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Related Concept Videos

Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:

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Related Experiment Video

Updated: Jun 18, 2026

Application of Long-term cultured Interferon-&#947; Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
15:57

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

Published on: July 11, 2015

Correlation between lymph node pathology and chemokine expression during bovine tuberculosis.

Stephanie Widdison1, Michael Watson, Tracey J Coffey

  • 1Bovine Genomics Group, Institute for Animal Health, Compton, Berkshire, RG20 7NN, UK. stephanie.widdison@bbsrc.ac.uk

Tuberculosis (Edinburgh, Scotland)
|November 21, 2009
PubMed
Summary

Chemokine expression in cattle lymph nodes increases with Mycobacterium bovis infection, correlating with disease severity. Vaccination reduces this chemokine response and disease progression in bovine tuberculosis.

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Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
12:53

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Published on: May 19, 2018

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Last Updated: Jun 18, 2026

Application of Long-term cultured Interferon-&#947; Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
15:57

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

Published on: July 11, 2015

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
12:53

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Published on: May 19, 2018

Area of Science:

  • Veterinary Immunology
  • Infectious Diseases
  • Molecular Biology

Background:

  • Bovine tuberculosis (bTB) is a significant global disease with limited understanding of host chemokine responses.
  • Chemokines play a crucial role in immune cell trafficking and regulating inflammatory responses during infection.

Purpose of the Study:

  • To investigate chemokine expression levels in lymph nodes of cattle with established Mycobacterium bovis infection.
  • To correlate chemokine expression with disease pathology and vaccination status.

Main Methods:

  • Quantification of chemokine and chemokine receptor expression in lymph node tissues from infected cattle.
  • Correlation analysis between chemokine levels, receptor expression, and pathological findings.
  • Comparison of chemokine profiles between vaccinated and unvaccinated infected animals.

Main Results:

  • Increased expression of several chemokines and their receptors was observed in infected cattle lymph nodes.
  • Significant correlations were found between specific chemokines and lymph node pathology, indicating a direct link to disease severity.
  • Vaccinated animals exhibited lower chemokine expression and reduced disease levels compared to unvaccinated controls.
  • The observed chemokine profile suggests a pro-inflammatory bias within the lymph node.

Conclusions:

  • Chemokine expression is elevated during established bovine tuberculosis and is directly associated with disease pathology.
  • Vaccination modulates the chemokine response, leading to reduced disease severity.
  • Ongoing chemokine expression by granuloma-associated cells likely contributes to sustained immune cell recruitment for infection control.