Multienzymatic system immobilization in sol-gel slides: fluorescent superoxide biosensors development

Area of Science:

• Biochemistry
• Analytical Chemistry
• Materials Science

Background:

• Biosensor development relies on immobilized multienzymatic systems for analytical applications.
• Superoxide radicals play a role in various biological processes and oxidative stress.

Purpose of the Study:

• To develop and evaluate analytical systems for superoxide radical detection using immobilized enzymes.
• To assess the capability of these biosensors in quantifying superoxide and evaluating antioxidant properties.

Main Methods:

• Utilized sol-gel technology for immobilizing enzyme systems.
• Developed three distinct analytical systems with varying enzyme immobilization strategies (two or three enzymes on single or multiple sol-gel slides).
• Employed a multienzyme cascade involving xanthine oxidase, superoxide dismutase, and horseradish peroxidase, coupled with Amplex Red detection.

Main Results:

• Successfully developed three analytical systems for superoxide radical detection.
• Demonstrated the potential of these systems to quantify superoxide radical levels.
• Evaluated the radical scavenging properties of various antioxidant compounds using the developed biosensors.

Conclusions:

• Sol-gel immobilized multienzymatic systems offer a promising approach for superoxide radical detection.
• The developed biosensors are suitable for quantifying superoxide and assessing antioxidant activity.
• This technology holds potential for applications in oxidative stress research and drug discovery.