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Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
Published on: February 21, 2018
Per2 is a C/EBP target gene implicated in myeloid leukemia
Sigal Gery1, H Phillip Koeffler
1Division of Hematology/Oncology, Cedars-Sinai Medical Center, UCLA School of Medicine, Los Angeles, CA 90048, USA.
Integrative Cancer Therapies
|November 21, 2009
Summary
Circadian rhythm disruptions linked to cancer may involve CCAAT/enhancer-binding proteins (C/EBPs). C/EBPs alter circadian genes like Per2, which shows tumor-suppressive effects in hematopoietic cancers.
Area of Science:
- Molecular Biology
- Chronobiology
- Oncology
Background:
- Circadian rhythms regulate essential physiological processes.
- Disruptions in circadian rhythms are linked to diseases including cancer.
- CCAAT/enhancer-binding proteins (C/EBPs) are transcription factors involved in cell growth and cancer.
Purpose of the Study:
- To investigate the relationship between C/EBPs and circadian clock components.
- To explore the role of circadian gene Per2 in hematopoietic cancers.
Main Methods:
- Expression profiling studies to identify altered circadian gene levels.
- Analysis of Per2 levels in patient samples and cell lines.
- Overexpression of Per2 in cancer cell lines to assess its functional impact.
Main Results:
- C/EBPs were found to significantly alter the levels of circadian genes Per2 and Rev-Erb alpha.
- Per2 levels were reduced in lymphoma and acute myeloid leukemia samples and cell lines.
- Per2 overexpression inhibited growth, induced cell cycle arrest, apoptosis, and reduced clonogenic ability in hematopoietic cancer cells.
Conclusions:
- C/EBPs influence circadian gene expression.
- Reduced Per2 levels are associated with hematopoietic cancers.
- Per2 exhibits tumor-suppressive properties, highlighting the role of circadian genes in cancer prevention.
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