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Updated: Jun 18, 2026

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters
Published on: April 20, 2015
The ClC-3 Cl-/H+ antiporter becomes uncoupled at low extracellular pH
James J Matsuda1, Mohammed S Filali, Malia M Collins
1Department of Pediatrics, University of Iowa Children's Hospital, Iowa City, Iowa 52242, USA.
Abstract:
Adenovirus expressing ClC-3 (Ad-ClC-3) induces Cl(-)/H(+) antiport current (I(ClC-3)) in HEK293 cells. The outward rectification and time dependence of I(ClC-3) closely resemble an endogenous HEK293 cell acid-activated Cl(-) current (ICl(acid)) seen at extracellular pH
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