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Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
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Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
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Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation
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Trypsin is a multifunctional factor in spermatogenesis.

Chiemi Miura1, Takashi Ohta, Yuichi Ozaki

  • 1Research Group for Reproductive Physiology, Southern Ehime Fisheries Research Center, Ehime University, 1289-1 Funakoshi, Ainan, Ehime 798-4292, Japan.

Proceedings of the National Academy of Sciences of the United States of America
|November 21, 2009
PubMed
Summary
This summary is machine-generated.

Trypsin, a pancreatic enzyme, plays a crucial role in fish spermatogenesis, including meiosis initiation and sperm development. This study reveals trypsin

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Published on: December 28, 2021

Area of Science:

  • Reproductive Biology
  • Enzymology
  • Fish Biology

Background:

  • Trypsin is primarily known as a digestive enzyme secreted by the pancreas.
  • The role of trypsin in reproductive processes, specifically spermatogenesis, is not well-established.
  • Progestins, like 17alpha,20beta-dihydroxy-4-pregnen-3-one (DHP), are known to regulate spermatogenesis in teleost fish.

Purpose of the Study:

  • To investigate the role of trypsin in the DHP-induced spermatogenesis pathway in teleost fish.
  • To determine the specific functions of trypsin during different stages of sperm development.
  • To explore the potential involvement of trypsin in fish fertilization.

Main Methods:

  • Upregulation of eel trypsinogen in eel testis following DHP treatment was analyzed.
  • In vitro eel testicular culture system was used to study trypsin's effects.
  • Trypsin activity was inhibited using specific antibodies and serine protease inhibitors.
  • Effects of varying trypsin doses on germ cell DNA synthesis, meiosis marker (Spo11) expression, and spermiogenesis were assessed.
  • Localization of trypsin in spermatozoa membranes was examined.

Main Results:

  • DHP treatment upregulated trypsinogen expression in eel testis, specifically in Sertoli cells and germ cell membranes.
  • Inhibition of trypsin significantly impaired DHP-induced spermatogenesis.
  • Low doses of trypsin stimulated DNA synthesis and Spo11 expression in germ cells, indicating a role in meiosis initiation.
  • Higher doses of trypsin induced spermiogenesis.
  • Trypsin was detected on spermatozoa membranes and associated with fertilization.

Conclusions:

  • Trypsin is an essential multifunctional factor in teleost fish spermatogenesis, regulating key events from meiosis initiation to spermiogenesis.
  • Trypsin's presence on spermatozoa suggests a role in fertilization.
  • These findings expand the known functions of trypsin beyond digestion into reproductive biology.