Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Nuclear Overhauser Enhancement (NOE)01:06

Nuclear Overhauser Enhancement (NOE)

Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling. This phenomenon, called the nuclear Overhauser enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring spin-active...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surface SO<sub>x</sub> Species Stabilized Metal-Oxygen Bonds in PtNi Nanoalloy for Highly Efficient and Durable Seawater Hydrogen Production.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

2D Materials Powering Neuromorphic Intelligence.

Nano-micro letters·2026
Same author

Kinetic vs Thermodynamic Processes in the Two-Dimensional Crystallization of a Hydrogen-Bonding Building Block.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Interfacial Reaction Competition in NiO/SiC for High-Performance UV Photodetection.

ACS nano·2026
Same author

Multi-Scale Charge Transfer in Organic Electroluminescence.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Sustainable and Scalable Flow Photochemical Conversions Using a Labile Ligand-Assembled Iron Complex.

JACS Au·2026

Related Experiment Video

Updated: Jun 18, 2026

Proximity Ligand Assay to Localize Proteins in DNA Damage Sites
09:39

Proximity Ligand Assay to Localize Proteins in DNA Damage Sites

Published on: August 2, 2024

Inducing nonlocal reactions with a local probe.

Jennifer M MacLeod1, Josh Lipton-Duffin, Chaoying Fu

  • 1Dipartimento di Fisicà, Universita degli Studi di Trieste, via Valerio 2, Trieste, TS, Italy.

ACS Nano
|November 26, 2009
PubMed
Summary
This summary is machine-generated.

Scanning tunneling microscopy (STM) now injects hot electrons to modify molecules remotely. This technique enables chemical changes up to 100 nm from the tip, opening new surface science applications.

More Related Videos

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

The Microfluidic Probe: Operation and Use for Localized Surface Processing
08:07

The Microfluidic Probe: Operation and Use for Localized Surface Processing

Published on: June 4, 2009

Related Experiment Videos

Last Updated: Jun 18, 2026

Proximity Ligand Assay to Localize Proteins in DNA Damage Sites
09:39

Proximity Ligand Assay to Localize Proteins in DNA Damage Sites

Published on: August 2, 2024

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
10:49

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

The Microfluidic Probe: Operation and Use for Localized Surface Processing
08:07

The Microfluidic Probe: Operation and Use for Localized Surface Processing

Published on: June 4, 2009

Area of Science:

  • Surface Science
  • Nanotechnology
  • Physical Chemistry

Background:

  • The scanning tunneling microscope (STM) has advanced beyond imaging to manipulation and chemical reactions.
  • Recent developments include injecting hot electrons into substrate surface states.

Discussion:

  • Injected electrons travel laterally, inducing chemical structure changes in molecules up to 100 nm away.
  • This phenomenon demonstrates a novel remote chemical modification capability.

Key Insights:

  • STM-injected hot electrons can induce localized chemical reactions at a distance.
  • Demonstrations show the feasibility of this remote electron injection technique.

Outlook:

  • Potential applications include characterizing interface states and controlled molecular patterning.
  • This technique offers new avenues for surface engineering and molecular assembly.