Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A software framework enabling analysis of plate-based flow cytometry data for high-throughput screening.

Assay and drug development technologies·2009
Same author

Fully human monoclonal antibodies antagonizing the glucagon receptor improve glucose homeostasis in mice and monkeys.

The Journal of pharmacology and experimental therapeutics·2009
Same author

CSMD1 is a novel multiple domain complement-regulatory protein highly expressed in the central nervous system and epithelial tissues.

Journal of immunology (Baltimore, Md. : 1950)·2006
See all related articles

Related Experiment Video

Updated: Jun 18, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Published on: June 28, 2017

Moving pictures: imaging flow cytometry for drug development.

Gary S Elliott1

  • 1Department of High Throughput Screening Molecular Pharmacology, Amgen Inc., One Amgen Center Dr, Thousand Oaks, CA 91320, USA. gelliott@amgen.com

Combinatorial Chemistry & High Throughput Screening
|November 26, 2009
PubMed
Summary

New imaging flow cytometry platforms offer quantitative cellular imaging for lead compound identification and profiling. This technology enables precise analysis of cellular behaviors, enhancing drug discovery screening capabilities.

More Related Videos

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
08:35

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

Published on: January 7, 2019

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking
09:01

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking

Published on: October 29, 2018

Related Experiment Videos

Last Updated: Jun 18, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Published on: June 28, 2017

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
08:35

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

Published on: January 7, 2019

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking
09:01

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking

Published on: October 29, 2018

Area of Science:

  • Biotechnology
  • Cell Biology
  • Drug Discovery

Background:

  • High throughput and high content screening technologies are advancing.
  • Quantitative cellular imaging is crucial for identifying and profiling lead compounds.
  • Accurate imaging and data enumeration are essential for statistical relevance in compound effect analysis.

Purpose of the Study:

  • To review the capabilities of the ImageStream imaging cytometer.
  • To discuss applications of imaging flow cytometry in compound screening and profiling.

Main Methods:

  • Imaging flow cytometry combines features of flow cytometry, microscopy, and imaging.
  • The ImageStream instrument provides highly quantitative analysis of cellular behaviors.
  • The technology supports highly multiplexed assays.

Main Results:

  • Imaging flow cytometry enables quantitative analysis of receptor internalization, phagocytosis, cell-cell communication, apoptosis, and nuclear translocation.
  • The ImageStream system offers enhanced capabilities for cellular behavior analysis.
  • The technology is relevant for compound screening and profiling.

Conclusions:

  • Imaging flow cytometry is a powerful tool for quantitative cellular imaging in drug discovery.
  • The ImageStream offers advanced capabilities for analyzing cellular responses to compounds.
  • This technology enhances the ability to screen and profile lead compounds effectively.