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Updated: Aug 14, 2026

Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context
Published on: October 13, 2011
Predominance of type III over type I cell surface procollagens in rat lung fibroblasts
1Department of Biochemistry, College of Medicine, University of Vermont, Burlington 05405.
Abstract:
Binding assays of 125I-labeled affinity-purified monospecific type I and III procollagen antibodies to fibroblasts have revealed the presence of type I and III procollagens on the cell surface, and that the amount of type III procollagen is 10 times greater than that of type I procollagen. Analyses by flow cytometry and fluorescent microscopy after treatment with an isothiocyanate (FITC)-conjugated second antibody supported this finding.
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