Related Experiment Video
Updated: Jun 18, 2026

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs
Published on: January 27, 2021
Influencing emulsifying properties of egg yolk by enzymatic modification with phospholipase D. Part 2: structural
Waldemar Buxmann1, Ute Bindrich, Ulf Strijowski
1German Institute of Food Technologies, Prof.-von-Klitzing-Str. 7, Quakenbrueck, Germany. w.buxmann@dil-ev.de
Abstract:
The effects of a direct incubation of egg yolk (EY) by phospholipase D (PLD) with respect to EY structure and interface properties of oil-in-water (o/w)-emulsions prepared with this EY were investigated. PLD incubation of EY mainly converts phosphatidylcholine into phosphatidic acid (PA). EY structures were characterised using circular dichroism and scanning electron microscopy. Composition (protein and phospholipid contents), charge, and appearance of interfaces in EY stabilised o/w-emulsions were described, too. The improved emulsifying activity of EY after PLD incubation could be attributed both to a higher interface activity of the PA itself and to modified interactions of protein and phospholipids in EY. PA possesses a negative charge resulting in droplet interfaces with higher charge. This effect increased repulsion forces between droplets compared to application of EY without PLD incubation. A higher portion of alpha-helix structures could be detected in the PLD-incubated EY enabling a higher flexibility of proteins on droplet surfaces. Additionally, a better preservation of EY protein structures during heat treatment due to the presence of PA enabled a higher thermal stability of such emulsions.
More Related Videos
11:59Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
Published on: May 15, 2019
12:18Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
Published on: August 3, 2021
Related Concept Videos
Membrane Fluidity
Membrane Fluidity
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is a relatively...
Asymmetric Lipid Bilayer
Synthesis of Phosphatidylcholine in the ER Membrane
The major components of all eukaryotic cell...
Overview of Fatty Acid Metabolism
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Lipid Digestion