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Comparison of FT4 with log TSH on the Abbott Architect ci8200: Pediatric reference intervals for free thyroxine and

Steven J Soldin1, Luke L Cheng, Lisa Y Lam

  • 1General Clinical Research Center, Georgetown University Medical Center, Washington, District of Columbia, USA. sjs44@georgetown.edu

Clinica Chimica Acta; International Journal of Clinical Chemistry
|November 26, 2009
PubMed

Insights

Serum free thyroxine (FT4) immunoassay on the Abbott Architect ci8200 shows poor clinical validity in children. Established pediatric reference intervals for FT4 and TSH are provided, with the Hoffmann method showing good agreement with the percentile approach.

Area of Science:

  • Pediatric Endocrinology
  • Clinical Chemistry
  • Immunoassay Development

Background:

  • Assessing the clinical validity of serum free thyroxine (FT4) measurements is crucial for accurate thyroid function assessment in children.
  • Establishing reliable pediatric reference intervals for FT4 and thyroid-stimulating hormone (TSH) is essential for the Abbott Architect ci8200 integrated system.

Purpose of the Study:

  • To evaluate the clinical validity of serum FT4 measurements by assessing its correlation with log TSH in a pediatric population.
  • To establish pediatric reference intervals for FT4 and TSH using the Abbott Architect ci8200.
  • To compare the percentile and Hoffmann approaches for deriving reference intervals.

Main Methods:

  • A population-based study involving 6023 children (3369 females, 2654 males).
  • Analysis of serum FT4 and TSH levels.
  • Comparison of percentile and Hoffmann methods for reference interval determination.

Main Results:

  • A poor correlation was observed between FT4 and log TSH (r=0.010 for males, r=0.050 for females).
  • Pediatric reference intervals for FT4 and TSH were established.
  • No significant sex differences were found for TSH and FT4; however, intervals decreased with age for both analytes.
  • The Hoffmann approach for reference intervals showed good agreement with the percentile approach.

Conclusions:

  • The FT4 immunoassay on the Abbott Architect ci8200 demonstrates limited clinical utility in the pediatric population due to poor FT4 vs. log TSH correlation.
  • Established pediatric reference intervals provide valuable data for the Abbott Architect ci8200.
  • The Hoffmann method is a viable alternative for establishing pediatric reference intervals, comparable to the percentile approach.
Abstract

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