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Updated: Jun 18, 2026

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
Published on: October 20, 2019
Coexistence of diploids and triploids in a Saharan relict olive: evidence from nuclear microsatellite and flow
Guillaume Besnard1, Djamel Baali-Cherif
1Imperial College London, Silwood Park Campus, Buckhurst Road, Ascot, Berkshire, SL5 7PY, UK. g.besnard@imperial.ac.uk
Abstract:
Polyploidy was recently reported in two endangered olive subspecies from North-West Africa. The origin of this phenomenon remains unclear. In the present study, 106 genotypes of a relict Laperrine's olive population form Hoggar (Algeria) were analysed using eight nuclear microsatellites. Diploid and triploid genetic profiles were observed. A flow cytometry analysis confirmed that trees displaying three alleles at several loci are effectively triploid. This report constitutes the first evidence for the coexistence of two ploidy types in an olive population. The triploid genotypes, probably in mean more vigorous than diploid trees, may be positively selected in the absence of sexual regeneration since a very long time, explaining their relative high frequency in the investigated population.
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