Related Experiment Video
Updated: Jun 18, 2026

Measurements of Physiological Stress Responses in C. Elegans
Published on: May 21, 2020
O-GlcNAcylation enhances FOXO4 transcriptional regulation in response to stress
Shiuh-Rong Ho1, Kai Wang, Thomas R Whisenhunt
1Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Abstract:
The FOXO4 transcription factor plays an important role in cell survival in response to oxidative stress. The regulation of FOXO4 is orchestrated by post-translational modifications including phosphorylation, acetylation, and ubiquitination. Here, we demonstrate that O-GlcNAcylation also contributes to the FOXO4-dependent oxidative stress response. We show that hydrogen peroxide treatment of HEK293 cells increases FOXO4 association with OGT, the enzyme that adds O-GlcNAc to proteins, causing FOXO4 O-GlcNAcylation and enhanced transcriptional activity under acute oxidative stress. O-GlcNAcylation is known to be protective for cells under stress conditions, including oxidative stress. Our data provide a mechanism of FOXO4 anti-oxidative protection through O-GlcNAcylation.
Related Concept Videos
Master Transcription Regulators
Stress Response System
Alarm stage
In the alarm stage, the body's initial...
Regulation of the Unfolded Protein Response
NF-kB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Other Stress Responses in Bacteria

