Related Experiment Video
Updated: Jun 18, 2026
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Published on: March 26, 2018
Acute myelogenous leukemia cells with the MLL-ELL translocation convert morphologically and functionally into
Haruko Tashiro1, Mitsuho Mizutani-Noguchi, Ryosuke Shirasaki
1Department of Hematology/Oncology, Teikyo University School of Medicine, Itabashi-ku, Tokyo 173-8606, Japan.
Abstract:
Bone marrow-myofibroblasts, a major component of bone marrow-stroma, are reported to originate from hematopoietic stem cells. We show in this paper that non-adherent leukemia blasts can change into myofibroblasts. When myeloblasts from two cases of acute myelogenous leukemia with a fusion product comprising mixed lineage leukemia and RNA polymerase II elongation factor, were cultured long term, their morphology changed to that of myofibroblasts with similar molecular characteristics to the parental myeloblasts. The original leukemia blasts, when cultured on the leukemia blast-derived myofibroblasts, grew extensively. Leukemia blasts can create their own microenvironment for proliferation.
