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Related Experiment Video

Updated: Jun 18, 2026

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

Multiple cyclophilins involved in different cellular pathways mediate HCV replication.

L Alex Gaither¹, Jason Borawski, Leah J Anderson

¹Novartis Institutes of Biomedical Research, Cambridge, MA 02139, USA.

|November 26, 2009

Summary

This summary is machine-generated.

Hepatitis C drug NIM811 inhibits viral replication by targeting multiple cyclophilins and cellular pathways, particularly protein trafficking. Further research is needed to fully understand its mechanism of action.

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Last Updated: Jun 18, 2026

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A Protocol for Analyzing Hepatitis C Virus Replication

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

Area of Science:

Virology
Molecular Biology
Drug Discovery

Background:

Cyclophilin inhibitors like DEBIO-025, SCY635, and NIM811 are in clinical trials for hepatitis C.
The precise mechanism of action for these cyclophilin inhibitors is not fully elucidated.
Human cells express at least 16 cyclophilins involved in various cellular functions.

Purpose of the Study:

To identify specific cyclophilins crucial for hepatitis C virus (HCV) replication.
To elucidate the mechanism of action of NIM811 in inhibiting HCV.
To investigate the cellular pathways affected by NIM811.

Main Methods:

Large-scale siRNA screening to identify essential cyclophilins.
Chemoproteomic assays using cyclophilin-binding compounds.
mRNA profiling of cells containing HCV replicons.

Main Results:

Confirmed the role of cyclophilin A in HCV replication.
Identified additional cyclophilin-containing pathways involved in HCV.
NIM811 demonstrated inhibition of multiple cyclophilins and pathways.
Protein trafficking was identified as the most significantly impacted pathway by NIM811.

Conclusions:

NIM811 exerts its antiviral effect by inhibiting multiple cyclophilins.
The inhibition of protein trafficking is a key mechanism by which NIM811 reduces HCV replication.
These findings contribute to understanding cyclophilin inhibitor mechanisms for hepatitis C therapy.