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Updated: Jun 18, 2026

On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System
Published on: July 18, 2025
[Pathogenicity and genetic divergence of two isolates of microsporidia Nosema bombycis]
Min WANG1, Jin-Shan XU, Lin-Ling WANG
1Laboratory of Animal Biology, Chongqing Normal University, Chongqing 400047, China. wm923@163.com
Abstract:
Microsporidia Nosema bombycis is a fungal pathogen that causes epidemic pebrine disease in Bombyx mori. Two N. bombycis isolates were obtained from two areas in China and showed different pathogenicity after Spodoptera frugiperda Sf9 cell cultivation. The regions of rDNAs from different isolates were analyzed, suggesting no relationship between the genetic divergence and their geographic distributions. Further analysis showed that several copies of SSU rDNA units in N. bombycis were interrupted by a MITE-like transposon, indicating the complexity of genomic structure in Nosema bombycis.
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