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A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

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Differential target gene activation by the Staphylococcus aureus two-component system saeRS.

Markus Mainiero¹, Christiane Goerke, Tobias Geiger

¹Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Tübingen, Germany.

Journal of Bacteriology

|November 26, 2009

Summary

The Staphylococcus aureus SaePQRS system regulates virulence. A specific SaeS protein variation (SaeS(P)) activates class I genes, while SaeS(L) represses them, differing in SaeR phosphorylation needs.

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Area of Science:

Microbiology
Molecular Biology
Bacterial Pathogenesis

Background:

The saePQRS system in Staphylococcus aureus regulates critical virulence factors.
Strain Newman exhibits a unique SaeS protein variant (SaeS(P)) compared to other strains (SaeS(L)).

Purpose of the Study:

To investigate the impact of the SaeS polymorphism on sae target gene expression.
To elucidate the role of SaeR phosphorylation levels in differential gene activation.

Main Methods:

Analysis of saeS deletion and mutated SaeR phosphorylation site strains.
Single-copy integration of saePQRS(L) into strain Newman.
Utilizing inducible saeRS constructs.

Main Results:

SaeS(P) strongly activates class I virulence genes, while SaeS(L) represses them.
Class II genes are unaffected by the SaeS polymorphism.
High SaeR phosphorylation is essential for class I gene activation; low levels activate class II genes.
SaePQRS dosage and SaeRS expression do not drive typical growth phase-dependent gene expression.

Conclusions:

The SaeS polymorphism dictates virulence factor expression through differential SaeR phosphorylation.
SaePQRS system regulation is complex and not solely dependent on SaeRS dosage or growth phase.