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Related Experiment Video

Updated: Jun 18, 2026

Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals
07:07

Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals

Published on: July 10, 2015

A microfluidic wound-healing assay for quantifying endothelial cell migration.

Andries D van der Meer1, Kim Vermeul, André A Poot

  • 1Department of Polymer Chemistry and Biomaterials, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands.

American Journal of Physiology. Heart and Circulatory Physiology
|November 26, 2009
PubMed
Summary

This study shows microfluidic wound-healing assays effectively measure endothelial cell migration. This method offers advantages like controlled vascular endothelial growth factor (VEGF) gradients and shear stress application for vascular research.

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Area of Science:

  • Vascular Biology
  • Cell Migration Assays
  • Microfluidics in Biology

Background:

  • Endothelial cell migration is crucial for blood vessel formation and tissue repair.
  • Reliable laboratory assays are needed to study endothelial migration.
  • The conventional wound-healing assay is a standard method.

Purpose of the Study:

  • To evaluate the microfluidic wound-healing assay as a research tool for vascular science.
  • To compare its performance with the conventional wound-healing assay.
  • To explore unique capabilities of the microfluidic approach.

Main Methods:

  • Endothelial cells cultured in a microfluidic channel to form a monolayer.
  • Wound generation using trypsin in a fluid stream.

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Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay
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Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay

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A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration
08:55

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

Published on: March 17, 2018

Related Experiment Videos

Last Updated: Jun 18, 2026

Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals
07:07

Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals

Published on: July 10, 2015

Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay
09:16

Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay

Published on: May 31, 2024

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration
08:55

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration

Published on: March 17, 2018

  • Analysis of wound closure over time to quantify cell migration.
  • Application of vascular endothelial growth factor (VEGF) and shear stress.

    • Main Results:

    • Microfluidic assay showed a lower migration rate than conventional assay but responded similarly to VEGF.
    • Vascular endothelial growth factor (VEGF) gradient generation increased migration rate.
    • Applied shear stress also significantly increased endothelial cell migration rate.

    Conclusions:

    • The microfluidic wound-healing assay is a viable alternative to conventional methods for measuring endothelial cell migration.
    • This assay provides unique advantages, including controlled gradient generation and shear stress application.
    • It offers a powerful tool for studying vascular cell behavior in a controlled environment.