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Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

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Published on: July 11, 2016

Sphingobacterium shayense sp. nov., isolated from forest soil.

Xiang He¹, Ting Xiao¹, Haiju Kuang¹

¹College of Life Science and Technology of Xinjiang University, Urumqi, Xinjiang 830046, PR China.

International Journal of Systematic and Evolutionary Microbiology

|November 26, 2009

Summary

A novel bacterium, Sphingobacterium shayense sp. nov., was identified from a Chinese poplar forest soil sample. This strictly aerobic, Gram-negative bacterium expands the known diversity within the Sphingobacterium genus.

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Area of Science:

Microbiology
Bacterial Taxonomy
Environmental Microbiology

Background:

Soil microbial communities harbor diverse, yet uncharacterized, bacterial species.
The genus Sphingobacterium comprises Gram-negative bacteria with significant roles in various ecosystems.
Understanding bacterial diversity is crucial for ecological and biotechnological applications.

Purpose of the Study:

To characterize a novel bacterial isolate from a Populus euphratica forest soil sample.
To determine the phylogenetic and taxonomic placement of the novel isolate within the Sphingobacterium genus.
To propose a new species designation based on comprehensive polyphasic characterization.

Main Methods:

Polyphasic taxonomic approach including phenotypic and genotypic analyses.
16S rRNA gene sequence analysis for phylogenetic placement.
Determination of major fatty acids, G+C content, and optimal growth conditions.

Main Results:

The isolate HS39(T) is a Gram-negative, yellow, aerobic, rod-shaped bacterium.
Phylogenetic analysis showed HS39(T) belongs to the genus Sphingobacterium, with S. mizutaii as the closest relative (94.0% similarity).
Key chemotaxonomic features include major fatty acids (iso-C₁₅:₀, iso-C₁₇:₀ 3-OH, summed feature 3) and MK-7 quinone.

Conclusions:

Strain HS39(T) represents a novel species within the genus Sphingobacterium.
The proposed name for this new species is Sphingobacterium shayense sp. nov.
The type strain is HS39(T) (=CCTCC AB 209006(T) =NRRL B-59203(T)).