Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Helixor A enhances natural killer cell activity by induction of PAI-1 expression through the p38/MAPK signaling pathway.

International immunopharmacology·2025
Same author

Therapeutic Effects of TN13 Peptide on Acute Respiratory Distress Syndrome and Sepsis Models In Vivo.

Journal of clinical medicine·2025
Same author

Injectable Endoplasmin-Loaded Lipid Nanoparticles-Hydrogel Composite for Cartilage Regeneration.

Tissue engineering and regenerative medicine·2025
Same author

Enhanced antileukemic effect of NKp30b isoform of donor-derived NK cells infused after HLA-haploidentical allogeneic hematopoietic cell transplantation in high-risk AML and MDS.

Bone marrow transplantation·2025
Same author

Vitamin D<sub>3</sub> Upregulated Protein 1 Deficiency Promotes Azoxymethane/Dextran Sulfate Sodium-Induced Colorectal Carcinogenesis in Mice.

Cancers·2024
Same author

T-plastin contributes to epithelial-mesenchymal transition in human lung cancer cells through FAK/AKT/Slug axis signaling pathway.

BMB reports·2024

Related Experiment Video

Updated: Jun 18, 2026

A BW Reporter System for Studying Receptor-Ligand Interactions
06:05

A BW Reporter System for Studying Receptor-Ligand Interactions

Published on: January 7, 2019

RasGRP1 is required for human NK cell function.

Suk Hyung Lee1, Sohyun Yun, Jiwon Lee

  • 1Cell Therapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Yuseong-gu, Daejeon, Republic of Korea.

Journal of Immunology (Baltimore, Md. : 1950)
|November 26, 2009
PubMed
Summary

Ras guanyl nucleotide-releasing protein 1 (RasGRP1) is essential for natural killer (NK) cell effector functions. Suppressing RasGRP1 blocks NK cell cytotoxicity and cytokine production by inhibiting the Ras-MAPK pathway.

More Related Videos

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins
07:20

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

Published on: June 14, 2018

Related Experiment Videos

Last Updated: Jun 18, 2026

A BW Reporter System for Studying Receptor-Ligand Interactions
06:05

A BW Reporter System for Studying Receptor-Ligand Interactions

Published on: January 7, 2019

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins
07:20

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

Published on: June 14, 2018

Area of Science:

  • Immunology
  • Cell Signaling
  • Molecular Biology

Background:

  • NK activating receptors trigger signal transduction via phospholipase-gamma, diacylglycerol, and Ca(2+).
  • Ras guanyl nucleotide-releasing protein 1 (RasGRP1) is activated by diacylglycerol and Ca(2+) and is critical for T-cell receptor-mediated Ras-ERK activation.

Purpose of the Study:

  • To investigate the role of RasGRP1 in human NK cell effector functions.
  • To determine if RasGRP1 is involved in the Ras-MAPK pathway activation in NK cells.

Main Methods:

  • RasGRP1 expression was suppressed in human NK cells using RNA interference (knockdown).
  • NK cell cytotoxicity and cytokine production were measured.
  • Activation of Ras, ERK, and JNK was assessed biochemically.
  • Specific pharmacological inhibitors were used to block the Ras-MAPK pathway.

Main Results:

  • RasGRP1 knockdown significantly impaired ITAM-dependent cytokine production and NK cell cytotoxicity.
  • RasGRP1-knockdown NK cells exhibited reduced activation of Ras, ERK, and JNK.
  • The Ras-MAPK pathway activation was confirmed as indispensable for NK cell effector functions.

Conclusions:

  • Ras guanyl nucleotide-releasing protein 1 (RasGRP1) is required for activating the Ras-MAPK pathway, which is essential for NK cell effector functions.
  • RasGRP1 may serve as a crucial link between phospholipase-gamma activation and NK cell effector functions through the Ras-MAPK pathway.