Rapid, simple technique to extend depth of field in microneurosurgical images: technical note

To increase the extent of the depth of field and focal sharpness in highly magnified image series from cadaveric microneurosurgical anatomic study or live surgery photographs obtained with the operating microscope, providing a higher quality and more satisfactory photographic and imaging experience.

We used a computer workstation, CombineZM software (Alan Hadley, United Kingdom, www.hadleyweb.pwp.blueyonder.co.uk/), an injected cadaver head specimen, and an operating microscope equipped with a digital single-lens reflex camera. Fifteen images were obtained of the dissection area through an anterior petrosal approach. The focus point was fixed to different points in each image. The images were loaded into CombineZM software for processing.

The stacking process of photographs with CombineZM freeware provides significant increase in extent of depth of field and wider area of image clarity, producing a sharp, high-quality image.

An image processed from a stack of photographs from cadaveric microneurosurgical studies or from the operating microscope in live surgery can be rendered to show extended 3-dimensional depth of field and clarity. This method offers improvements for editing, displaying, and publishing neurosurgical anatomic images.