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Updated: Jun 18, 2026

Aortic Ring Assay

Aortic Ring Assay

Published on: November 24, 2009

Aortic ring assay.

Keren Bellacen¹, Eli C Lewis

¹Department Clinical Biochemistry, Ben-Gurion University of the Negev.

Journal of Visualized Experiments : Jove

|November 26, 2009

Summary

This summary is machine-generated.

The aortic ring assay models blood vessel growth (angiogenesis) using mouse aorta segments in culture. This method allows studying angiogenic factors and inhibitors in a complex tissue environment.

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Aortic Ring Assay

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Published on: November 24, 2009

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Area of Science:

Biomedical Engineering
Developmental Biology
Vascular Biology

Background:

Angiogenesis, the formation of new blood vessels, is crucial for both physiological and pathological processes.
Studying angiogenesis often involves endothelial cells in culture, but organ culture models offer a more complex environment.
The aortic ring assay provides an in vitro model for observing angiogenesis from a native tissue source.

Purpose of the Study:

To describe and validate the aortic ring assay as a model for studying angiogenesis.
To enable the investigation of angiogenic factors and inhibitors.
To facilitate the study of paracrine effects in angiogenesis using co-cultures.

Main Methods:

Mouse thoracic aortas are excised, cleaned, and sectioned into ~1 mm rings.
Aortic rings are embedded in basement matrix extract (BME) within a 48-well plate.
Neovessel outgrowth is monitored over 6-12 days using microscopy; supernatants are collected for molecular analysis.

Main Results:

The aortic ring assay successfully models neovessel outgrowth from aortic segments.
The assay allows for the direct application of angiogenic factors and inhibitors.
Co-culture with other cell types enables the study of paracrine signaling in angiogenesis.

Conclusions:

The aortic ring assay is a versatile organ culture model for studying angiogenesis.
This method is suitable for evaluating the effects of various compounds on blood vessel formation.
The assay provides a platform for investigating complex cellular interactions in vascular development.